For Research Use Only

Cryo-Ess™
Animal Component Free Cryopreservation medium

Eliminate DMSO from Cryopreservation

• NON-TOXIC
• Animal Component Free
• No cryopreservation agents
• Does not crystallize or form ice during the freezing or thawing processes

Effective, Efficient Performance

• Save time and steps
• Performs better than DMSO without the toxic effects

Cryo-Ess™ has demonstrated cryopreservation of the following cell types:

• Platelets
• Bone marrow
• Cartilage
• Islet cells
• Cord blood cells
• Vero cells
• Chick embryo fibroblasts
• Human embryonic kidney (HEK)
• Equine stem cells
• Leucocytes (B&T cells)

	This graph shows the recovery rate of Vero Cells and Chick embryos cells after freezing. Cryo-Ess™ has a superior recovery rate over DMSO and Cryo-Ess™ is not toxic to the cells once they have thawed.

Applications

Cryo-Ess™ (Cryo-preservation medium) is used as a cryopreservation solution without addition of cryoprotective agents (e.g. DMSO. HES).

Using a controlled method of freezing tissues or cells, they can be stored down to -196° in Cryo-Ess™ without adding DMSO, where they are maintained in a vital state. Crystallization of liquid in the tissue or cells is minimized in Cryo-Ess™ during thawing (which is critical for cell viability). Therefore tissue and cells in the tissue will remain vital in thawing in Cryo-Ess™.

Product Description

Cryo-Ess™ is for RESEARCH USE ONLY.
Cryo-Ess™ is serum and animal component free.

Storage and Stability

Store Cryo-Ess™ at 2° to 8° C (refrigeration) away from light.
24 months stability.

Jurkat Cells Frozen in Cryo-Ess™ Freezing Medium

	Results of two independent freezing experiments. Jurkat cells were frozen in CryoEssTM freezing medium and as control in 90% cell culture medium + 10% DMSO (standard). Directly after thawing the cells were counted and the viability was measured by the automated cell counter CASY TT (direct viability).

Click here for the Cryo-Ess Material Safety Data Sheet (MSDS).