Recently Essential Pharmaceuticals wrote a guest blog for the Cell Culture Dish entitled "Increase Protein Yield by Striking the Right Balance between Cell Density and Protein Productivity." It was published on August 3rd and can be read in its entirety here.
The blog investigates the relationship between viable cell density and protein yield with the help of a study evaluating cell metabolism in fed-batch CHO cell culture. Here is an excerpt:
The study shows the relationship between declining biomass (or less total cell density) and an increase in monoclonal antibody. The late stage of the cell lifecycle is when the CHO cell is a highly productive protein factory. Associated with those different stages of the life cycle are different resource needs observed over the bioproduction run. In effect, active proliferation and increasing cell density is counter to protein productivity. Therefore, fast proliferation and increased VCD may not correlate with an increased protein yield.
To read more about the relationship between cell density and protein yield, download our Did You Know piece discussing the same topic.
Stay tuned for more postings on the Cell Culture Dish blog.