FBS Compounds and Contaminants

ITEM

FBS CONTENT

CELL-ESS® CONTENT

Adventitious Agents: There are many infectious agents in serum which must be screened and can affect your results.

Bovine Adeno 3 Virus (BAV-3)

Test required

Zero – no animal components

Bovine Parvovirus (BPV)

Test required

Zero – no animal components

Bovine Respiratory Syncytial Virus

Test required

Zero – no animal components

Bovine Viral Diarrhea Virus (BVDV)

Test required

Zero – no animal components

Endotoxin

Test required

Zero – no animal components

Mycoplasma

Test required

Zero – no animal components

Parainfluenza-3 Virus (PI-3V)

Test required

Zero – no animal components

Prions

Test required

Zero – no animal components

Rabies virus

Test required

Zero – no animal components

REO Virus

Test required

Zero – no animal components

Hormones:  Can influence systemic changes and be tightly regulated in cellular work, but levels in FBS are highly variable and often untested.

Cortisol

4.0 – 5.5nM

Zero

Estrogen

77 – 81 ppm

Zero

Insulin

31 – 48 ppm

Zero

LDH (Lactate Dehydrogenase Hormone)

344 – 465 U/L

Zero

Progesterone

0.1 – 0.3 nM

Zero

T3 Tyroid Hormone

0.9 – 1.2 nM

Zero

T4 (Thyroxine)

129 – 200 nM

Zero

Testosterone

0.2 – 1.1 nM

Zero

Attachment Factors: Can cause non-specific adherence of cells to plastics resulting in morphological changes and functional changes.

Collagen

Widely variable and untested

Zero

Fibronectin

Widely variable and untested

Zero

Keratin

Widely variable and untested

Zero

Vitronectin

Widely variable and untested

Zero

Signaling Molecules: FBS contains varying levels of these potent signaling molecules which can cause a array of effects on responding cells.

Histamine

Widely variable and untested

Zero

HMGB1

Widely variable and untested

Zero

Integrin

Widely variable and untested

Zero

Retinoic Acid

Widely variable and untested

Zero

TGF-Beta

Widely variable and untested

Zero

Other: There are dozens of proteins in FBS which have a wide array of effects causing large variability in lot-to-lot results.

Alkaline Phosphatase

123 – 259 U/L

Zero

ALT (Ananine Transaminase)

3 – 6 U/L

Zero

AST (Asparate Aminotranferase)

23 – 39 U/L

Zero

Bovine Albumin

11 – 34g/L

Zero

Bilirubin

0.2 – 0.3mg/dL

Zero

Creatinine

2.5 – 2.6mg/dL

Zero

GGT (Gamma-glutamyl Transferase)

4 – 6 U/L

Zero

Hemoglobin

27 – 200mg/L

Zero

IgG

83 – 105mg/L

Zero

Trigylcerides

41 – 50mg/dL

Zero

Urea

3.7 – 4.8mM

Zero

Total Protein

34 – 42g/L

< 40 mg/L