Bioscience Jena
Lab Reagents
Human IgG antibody Laboratories manufactures the bioscience jena reagents distributed by Genprice. The Bioscience Jena reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. To purchase these products, for the MSDS, Data Sheet, protocol, storage conditions/temperature or for the concentration, please contact Jena Bioscience GmbH. Other Bioscience products are available in stock. Specificity: Bioscience Category: Jena
HDAC Fluorogenic Assay Kit |
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50033 | BPS Bioscience | 96 rxns. | EUR 445 |
Description: The Fluorogenic HDAC Assay Kit is a complete assay system_x000D_designed to measure histone deacetylase (HDAC) class 1 activity for screening and_x000D_profiling applications. It comes in a convenient 96-well format, with all the reagents_x000D_necessary for 100 fluorescent HDAC activity measurements. In addition, the kit_x000D_includes purified HDAC2 enzyme and a potent HDAC inhibitor, Trichostatin A,_x000D_for use as a positive and negative control. The Fluorogenic HDAC Assay_x000D_Kit is based on a unique fluorogenic substrate and developer combination. This assay_x000D_method eliminates dealing with the radioactivity, extraction, and chromatography aspects_x000D_of traditional assays. Using this kit, only two simple steps on a microtiter plate are needed_x000D_to analyze the HDAC activity level. First, the HDAC fluorometric substrate, containing_x000D_an acetylated lysine side chain, is incubated with purified HDAC enzyme._x000D_The deacetylation sensitizes the substrate so subsequent treatment with the Lysine_x000D_Developer produces a fluorophore that can then be measured using a fluorescence reader. |
HDAC Fluorogenic Assay Kit (Green) |
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50034 | BPS Bioscience | 96 rxns. | EUR 450 |
Description: The Fluorogenic HDAC Assay Kit is a complete assay system_x000D_designed to measure histone deacetylase (HDAC) class 1 activity for screening_x000D_and profiling applications. The kit comes in a convenient 96-well format, with all the_x000D_reagents necessary for 100 fluorescent HDAC activity measurements. In_x000D_addition, the kit includes purified HDAC2 enzyme and a potent HDAC inhibitor,_x000D_Trichostatin A, for use as a positive and negative control. The Fluorogenic HDAC_x000D_Assay Kit is based on a unique fluorogenic substrate and developer combination._x000D_This assay method eliminates dealing with the radioactivity, extraction, and_x000D_chromatography aspects of traditional assays. Using this kit, only two simple_x000D_steps on a microtiter plate are needed to analyze the HDAC activity level. First,_x000D_the HDAC fluorometric substrate, containing an acetylated lysine side chain, is_x000D_incubated with purified HDAC enzyme. The deacetylation sensitizes the_x000D_substrate so subsequent treatment with the Lysine Developer produces a_x000D_fluorophore that can then be measured using a fluorescence reader at 485 nm_x000D_(excitation)/528 nm (emission)._x000D__x000D_Our other HDAC kit (#50033) contains a substrate that is excited at wavelengths 350-380 nm and fluoresces at wavelengths 440-460 nm. However, the substrate in this kit fluoresces at longer (green) wavelengths: 485 nm(excitation)/528 nm (emission). It is most useful when the sample or inhibitor fluoresces at wavelengths that overlap those of our other HDAC substrate |
Fluorogenic HDAC substrate 3 |
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50037 | BPS Bioscience | 250 nmole | EUR 255 |
Description: A fluorogenic, acetylated peptide substrate for class I HDACs (HDACs 1, 2, and 3) and class 2b HDACs (HDACs 6 and 10). This substrate fluoresces at excitation 350-380 nm, emission at 440-460 nm. If autofluorescence of the inhibitor near this wavelength is an issue, we recommend using Fluorogenic HDAC substrate 2 (green), BPS catalog #50038. For class 2a HDACs (HDACs 4, 5, 7, and 9) or HDACs 8 and 11, we recommend #50040. |
Fluorogenic HDAC Substrate 2 (Green fluorescence) |
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50038 | BPS Bioscience | 250 nmole | EUR 235 |
Description: A fluorogenic, acetylated peptide substrate for HDACs. It is a suitable substrate for class I HDACs (HDACs 1, 2, and 3) and class 2b HDACs (HDACs 6 and 10). This substrate exhibits longer excitation and emission wavelengths (excitation 485 nm, emission 528 nm) than our regular class 1/2b HDAC substrate, #50037, which fluoresces at excitation 350-380 nm, emission at 440-460 nm. This substrate should be used when the inhibitor fluoresces near 450 nm. If autofluorescence is not an issue, we recommend HDAC substrate 3, #50037. For autofluorescent samples using HDACs 4, 5, 7, 8, 9, and 11, we recommend substrate #50042. |
Fluorogenic HDAC class 2a substrate |
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50040 | BPS Bioscience | 250 nmole | EUR 265 |
Description: A fluorogenic, acetylated peptide substrate for class2a HDACs (HDACs 4, 5, 7, and 9). It also works well for HDACs 8 and 11. This substrate fluoresces at excitation 350-380 nm, emission at 440-460 nm. If autofluorescence of the inhibitor near this wavelength is an issue, we recommend using Fluorogenic HDAC class 2a substrate 2 (green), BPS catalog #50042. For HDACs 1-3 ,6, and 10, we recommend substrate #50037. |
Fluorogenic HDAC class 2a substrate 2 (green) |
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50042 | BPS Bioscience | 250 nmole | EUR 235 |
Description: A fluorogenic, acetylated peptide substrate for class2a HDACs (HDACs 4, 5, 7 and 9). It also works well for HDACs 8 and 11. This substrate exhibits longer excitation and emission wavelengths (excitation 485 nm, emission 528 nm) than our regular class 2a HDAC substrate, #50040, which fluoresces at excitation 350-380 nm, emission at 440-460 nm. This substrate should be used when the inhibitor fluoresces near 450 nm. If autofluorescence is not an issue, we recommend product #50040. For autofluorescent samples using HDACs 1-3 ,6, and 10, we recommend substrate #50038. |
HDAC5 (full length), GST-tag |
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50045 | BPS Bioscience | 5 µg | EUR 490 |
Description: Human HDAC5 , GenBank Accession No. NM_005474, full length with N-terminal GST-tag, MW= 150 kDa, expressed in baculovirus expression system. |
HDAC6 (H216A), GST-tag |
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50046 | BPS Bioscience | 50 µg | EUR 465 |
Description: Human HDAC6 with H216A mutation (GenBank Accession No. NM_006044), full length with N-terminal GST-tag, MW= 161 kDa, expressed in a baculovirus expression system. |
HDAC1, FLAG-tag, His-tag |
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50051 | BPS Bioscience | 50 µg | EUR 485 |
Description: Human HDAC1, GenBank Accession No. NM_004964, full length with C-terminal FLAG and C-terminal His-tag, MW= 56 kDa, expressed in baculovirus expression system. |
HDAC2, FLAG-tag |
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50052 | BPS Bioscience | 50 µg | EUR 440 |
Description: Human HDAC2 , GenBank Accession No. Q92769, full length with C-terminal FLAG-tag, MW= 60 kDa, expressed in baculovirus expression system. |
HDAC6, FLAG-tag |
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50056 | BPS Bioscience | 50 µg | EUR 495 |
Description: Human HDAC6 (GenBank Accession No. NM_006044), full length with C-terminal FLAG-tag, MW= 132 kDa, expressed in baculovirus expression system. |
HDAC6, GST-Tag (Mouse) |
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50057 | BPS Bioscience | 50 µg | EUR 440 |
Description: Mouse HDAC6, also known as histone deacetylase 6, GenBank Accession No. NM_010413, a.a. 2-1149 (end) with N-terminal GST-Tag, expressed in baculovirus expression system. MW = 152 kDa. |
HDAC1, His-Tag, FLAG-Tag (Mouse) |
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50058 | BPS Bioscience | 50 µg | EUR 440 |
Description: Mouse HDAC1, GenBank Accession No. NM_008228, amino acids 1-482 (full length), with C-terminal His-tag and C-terminal FLAG-tag, MW = 57 kDa, expressed in an Sf9 baculovirus expression system. Recombinant HDAC1 forms a complex with endogenous Hsp70. The identity of Hsp70 was confirmed by MALDI-TOF mass spectrometry. |
HDAC10, N-FLAG-tag |
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50060 | BPS Bioscience | 50 µg | EUR 410 |
Description: Human HDAC10 (a.a. 2-631) (GenBank Accession No. NM_032019) with N-terminal FLAG-tag, MW= 67 kDa, expressed in Sf9 cells via a baculovirus expression system. |
HDAC1 Fluorogenic Assay Kit |
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50061 | BPS Bioscience | 96 rxns. | EUR 465 |
Description: The Fluorogenic HDAC1 Assay Kit is a complete assay system_x000D_designed to measure histone deacetylase 1 (HDAC1) activity for screening and profiling_x000D_applications. It comes in a convenient 96-well format, with all the reagents necessary for_x000D_100 fluorescent HDAC1 activity measurements. In addition, the kit includes purified_x000D_HDAC1 enzyme and a potent HDAC inhibitor, Trichostatin A, for use as a positive and_x000D_negative control. The Fluorogenic HDAC1 Assay Kit is based on a unique fluorogenic_x000D_substrate and developer combination. This assay method eliminates dealing with the_x000D_radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit,_x000D_only two simple steps on a microtiter plate are needed to analyze the HDAC activity_x000D_level. First, the HDAC fluorometric substrate, containing an acetylated lysine side chain,_x000D_is incubated with purified HDAC1. The deacetylation sensitizes the substrate so_x000D_subsequent treatment with the Lysine Developer produces a fluorophore that can then_x000D_be measured using a fluorescence reader._x000D_ |
HDAC2 Fluorogenic Assay Kit |
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50062 | BPS Bioscience | 96 rxns. | EUR 445 |
Description: The Fluorogenic HDAC2 Assay Kit is a complete assay system_x000D_designed to measure histone deacetylase 2 (HDAC2) activity for screening and profiling_x000D_applications. It comes in a convenient 96-well format, with all the reagents necessary for_x000D_100 fluorescent HDAC2 activity measurements. In addition, the kit includes purified_x000D_HDAC2 enzyme and a potent HDAC inhibitor, Trichostatin A, for use as a positive and_x000D_negative control. The Fluorogenic HDAC2 Assay Kit is based on a unique fluorogenic_x000D_substrate and developer combination. This assay method eliminates dealing with the_x000D_radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit,_x000D_only two simple steps on a microtiter plate are needed to analyze the HDAC activity_x000D_level. First, the HDAC fluorometric substrate, containing an acetylated lysine side chain,_x000D_is incubated with purified HDAC2. The deacetylation sensitizes the substrate so_x000D_subsequent treatment with the Lysine Developer produces a fluorophore that can then_x000D_be measured using a fluorescence reader. |
pf-HDAC1, Malaria, His-tag, FLAG-tag |
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50063 | BPS Bioscience | 20 µg | EUR 440 |
Description: Malarial (Plasmodium falciparum) HDAC, Genbank Accession No. AAD22407.1, full length (1-450) with C-terminal His-tag and FLAG-tag, MW=53.4 kDa, expressed in a Baculovirus infected Sf9 cell expression system. |