Molecular farming in tobacco hairy roots by triggering the secretion of a pharmaceutical antibody.
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Molecular farming in tobacco hairy roots by triggering the secretion of a pharmaceutical antibody.
recombinant pharmaceutical proteins expressed in the culture of hair follicles can be secreted into the medium to improve the homogeneity of the product and to facilitate purification, although this could result in significant degradation if proteins are inherently unstable or susceptible to protease.
To address this challenge, we used the experimental design approach to develop a protocol optimized for the induction of hair follicles tobacco cultivation issued a full size monoclonal antibody M12. Results antibody enhanced 30-fold with the addition of 14 g / L KNO 3, 19 mg / L 1-naphthaleneacetic acid and 1.5 g / L of polyvinylpyrrolidone stabilization agent. Analysis of hairy root cross sections reveal that the optimized media induced lateral root formation and morphological changes in the cortex and Pericycle in cells, indicating that the increase in productivity of at least partly based on improved efficiency of antibody secretion.
We found that 57% of the antibody was removed, resulting in 5.9 mg of product per liter of induction medium. Both secreted and intracellular forms of antibodies can be isolated by protein A affinity chromatography and their functions are authenticated using vitronectin-binding test. glycan analysis reveals three main factory complex-type glycans in both forms of the antibodies, although the secreted form more homogeneous due to the dominance of certain glycoforms.
Therefore the tobacco hairy root culture to offer practical solutions for the production of pharmaceutical homogeneous antibody in detention. Such as biomarker discovery takes center stage, the role of immunohistochemistry in the process of rising. At the same time, the number of antibodies produced to “research use” continue to increase and it is important that the antibodies to be used as a biomarker validated for specificity and sensitivity prior to use.
These guidelines seek to provide a phased approach to the validation of antibodies for immunohistochemistry tests, reflecting the views of a consortium of academic researchers and Histopathological based pharmaceuticals. We propose that the antibody is placed into a tier system, the level of 1-3, based on the evidence of their use in immunohistochemistry, and that the level of validation that is required is proportional to where they were at that level.
Molecular farming in tobacco hairy roots by triggering the secretion of a pharmaceutical antibody.
Benchmarking B-cell epitope prediction data quantitative dose-response antibody antipeptida: the development of new pharmaceutical products.
B-cell epitope prediction can enable the development of new pharmaceutical products. However, consensus has not yet emerged mechanically framed on a comparison of these predictions, so it presents an opportunity to set the standard practice of inconsistencies epistemic avoid casting epitope prediction-task as a binary classification problem.
As an alternative to the benchmark data qualitative dichotomous conventional, quantitative data is the dose-response of the biological effects of antibody-mediated more meaningful from the perspective of information theory in the sense that these effects can be expressed as a probability (eg, inhibition of functional antibody) in which the information Shannon entropy (SIE ) can be evaluated as a measure of informativeness.
Pig Advanced Glycosylation End Product Specific Receptor (AGER) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Advanced Glycosylation End Product Specific Receptor (AGER) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Rat Advanced Glycosylation End Product Specific Receptor (AGER) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Advanced Glycosylation End Product Specific Receptor (AGER) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Rat Advanced Glycosylation End Product Specific Receptor (AGER) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Advanced Glycosylation End Product Specific Receptor (AGER) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Rat Advanced Glycosylation End Product Specific Receptor (AGER) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Advanced Glycosylation End Product Specific Receptor (AGER) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Rat Advanced Glycosylation End Product Specific Receptor (AGER) ELISA Kit
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Rat Advanced Glycosylation End Product Specific Receptor (AGER) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.
Rat Advanced Glycosylation End Product Specific Receptor (AGER) ELISA Kit
Description: A sandwich quantitative ELISA assay kit for detection of Human Advanced Glycosylation End Product Specific Receptor (AGER) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Human Advanced Glycosylation End Product Specific Receptor (AGER) ELISA Kit
Description: A sandwich quantitative ELISA assay kit for detection of Human Advanced Glycosylation End Product Specific Receptor (AGER) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Mouse Advanced Glycosylation End Product Specific Receptor (AGER) ELISA Kit
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Advanced Glycosylation End Product Specific Receptor (AGER) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Mouse Advanced Glycosylation End Product Specific Receptor (AGER) ELISA Kit
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Advanced Glycosylation End Product Specific Receptor (AGER) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Human Advanced Glycosylation End Product Specific Receptor (AGER) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Advanced Glycosylation End Product Specific Receptor (AGER) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Human Advanced Glycosylation End Product Specific Receptor (AGER) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Advanced Glycosylation End Product Specific Receptor (AGER) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Human Advanced Glycosylation End Product Specific Receptor (AGER) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Advanced Glycosylation End Product Specific Receptor (AGER) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Human Advanced Glycosylation End Product Specific Receptor (AGER) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Advanced Glycosylation End Product Specific Receptor (AGER) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Human Advanced Glycosylation End Product Specific Receptor (AGER) ELISA Kit
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Advanced Glycosylation End Product Specific Receptor (AGER) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.
Mouse Advanced Glycosylation End Product Specific Receptor (AGER) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Advanced Glycosylation End Product Specific Receptor (AGER) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Mouse Advanced Glycosylation End Product Specific Receptor (AGER) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Advanced Glycosylation End Product Specific Receptor (AGER) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Mouse Advanced Glycosylation End Product Specific Receptor (AGER) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Advanced Glycosylation End Product Specific Receptor (AGER) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Mouse Advanced Glycosylation End Product Specific Receptor (AGER) ELISA Kit
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Advanced Glycosylation End Product Specific Receptor (AGER) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Mouse Advanced Glycosylation End Product Specific Receptor (AGER) ELISA Kit
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mouse Advanced Glycosylation End Product Specific Receptor (AGER) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.
Plant Advanced Glycosylation End Product Specific Receptor (AGER) ELISA Kit
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat AGER. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat AGER. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat AGER, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat AGER in the samples is then determined by comparing the OD of the samples to the standard curve.
Rat AGER(Advanced Glycosylation End Product Specific Receptor) ELISA Kit
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat AGER. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat AGER. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat AGER, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat AGER in the samples is then determined by comparing the OD of the samples to the standard curve.
Rat AGER(Advanced Glycosylation End Product Specific Receptor) ELISA Kit
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human AGER. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human AGER. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human AGER, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human AGER in the samples is then determined by comparing the OD of the samples to the standard curve.
Human AGER(Advanced Glycosylation End Product Specific Receptor) ELISA Kit
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human AGER. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human AGER. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human AGER, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human AGER in the samples is then determined by comparing the OD of the samples to the standard curve.
Mouse AGER(Advanced Glycosylation End Product Specific Receptor) ELISA Kit
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse AGER. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse AGER. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse AGER, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse AGER in the samples is then determined by comparing the OD of the samples to the standard curve.
Mouse AGER(Advanced Glycosylation End Product Specific Receptor) ELISA Kit
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse AGER. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse AGER. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse AGER, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse AGER in the samples is then determined by comparing the OD of the samples to the standard curve.
Human Advanced Glycosylation End Product Specific Receptor ELISA Kit (AGER)
Thus the biological effect, half-maximum (for example, the median inhibitory concentration of antibodies) in accordance with the maximum data information, while the biological effects are not detected and the maximum in accordance with the minimum data.This informative apply for benchmarking epitope prediction B-cells for peptide-based design immunogens that antipeptida pose with the relevant functional antibody cross-reactivity.
